Al replicates. (XLS)Table S9 Immunostaining images from Human Protein Atlas.
(two) The ``Tion with partners, which is positively related with condom use.26 However tissue specificity spread sheet describes validation of your differentially coexpressed genes according to the GSE7307 dataset. For each gene up-regulated in the similar tissue exactly where the gene represents a ciliary hub, statistical significance of up-regulation is provided (Student's t-test P-value soon after a Benjamini-Hochberg correction). For genes supported as tissue-specific markers of ciliated cells, the underlying tissue specificity profile is visualized as a histogram. (three) The ``Protein Atlas spread sheet describes validation of the differentially coexpressed genes with immunostaining information.Al replicates. (XLS)Table S9 Immunostaining images from Human Protein Atlas. Proteins are grouped by novelty categories: recognized ciliary proteins (category I), previously predicted candidates (category II) and novel candidates (category III). The summary at the top with the document fnhum.2013.00596 briefly describes the staining pattern of every single protein inside the tissues and specifies the resulting staining class. The immunohistochemical photos underlying the protein classification are provided under the summary table. The ``Protein column specifies protein name, antibody ID and novelty category of each and every protein. The ``Airways and ``Fallopian tubes columns present Protein Atlas images for the respective tissues. Within the ``AirwaysDifferential coexpression evaluation involving the tissues. (1) The ``Differential coexpression spread sheet includes only the differentially coexpressed genes. For every single gene, the table specifies its membership (MM) inside the ciliary module in every dataset. Module membership was calculated as Pearson correlation among expression profile of a gene and integrated expression profile in the ciliary module (see Methods, section ``Expanding modules for the genome scale). Green indicates that the gene belongs for the journal.pone.0174724 ciliary module in a provided dataset. Genes that belong for the ciliary module and have MM value .0.75 were viewed as hubs inside the respective dataset. For any offered gene, statistical significance of MM difference among the tissues is described by ANOVA P-value. (2) The ``Tissue specificity spread sheet describes validation of your differentially coexpressed genes depending on the GSE7307 dataset. The table shows expression levels of your genes in ten ``ependyma-positive brain samples, 7 airway samples and three fallopian tube samples. For each gene, imply expression level inside the tissues is offered. Tissue with the highest expression level of each and every gene is colored determined by centrality status in the gene in this tissue. Specifically, green denotes that the gene represents a hub in this tissue's ciliary module, grey denotes that the gene represents a hub within a different tissue's ciliary module. For every gene up-regulated in the identical tissue exactly where the gene represents a ciliary hub, statistical significance of up-regulation is offered (Student's t-test P-value right after a Benjamini-Hochberg correction). For genes supported as tissue-specific markers of ciliated cells, the underlying tissue specificity profile is visualized as a histogram. (3) The ``Protein Atlas spread sheet describes validation of the differentially coexpressed genes with immunostaining data. Human Protein Atlas contained information for three in the 8 differentially coexpressed genes. Because ependyma is absent from Protein Atlas, we compared the immunostaining data in between airways (bronchus and nasopharynx) and fallopian tubes. (XLS)Table SAcknowledgmentsWe would like to thank Aliesa de Bray and Steven Kunnen in the Leiden University Health-related Center for performing the immunofluorescent staining of the MDCK cell line.Author ContributionsConceived and created the experiments: AI MS PTH DP WVR.