An be explained by 4E6's high affinity for solubilized PHF: Unterschied zwischen den Versionen

Antibodies with access to both intra- and extracellular pools of pathological tau protein are likely to be more [https://dx.doi.org/10.3389/fpsyg.2016.01503 title= fpsyg.2016.01503] [http://kfyst.com/comment/html/?244060.html Al irritation (including abdominal swelling, tenderness, sensitivity, stiffness, a lower] efficacious than antibodies acting only within one compartment.ResultsIn vivo studies 4E6 acutely improves cognition in htau mice and reduces soluble phospho-tau protein, whereas 6B2 does not affect cognition or tau levelsAt 11?2 months of age, the htau mice were assigned to control and treatment groups with similar cognitive (CFSM test) and motor status (Rotorod and Open Field). 2c ), suggesting that the 4E6-mediated improvements in cognitive functions were direct results of the immunization, but not secondary effects from motor function changes.Immunohistochemistry, western and immunoblot analysesRather mild tau pathology was detected in PHF-1 or MC1 stained brain sections (Additional file 1: Figure S1). PHF-1 stained sections had some neuropil staining but lacked cell body staining. Likewise, MC1 staining was limited, although a few intraneuronal tau aggregates were ev.An be explained by 4E6's high affinity for solubilized PHF, whereas the ineffective 6B2 binds primarily to aggregated but not to solubilized PHF. Hence, affinity for particular forms of tau predict efficacy. Further, whether the antibody is working outside or inside the neuron depends on the timing of PHF and antibody addition. Antibodies with access to both intra- and extracellular pools of pathological tau protein are likely to be more [https://dx.doi.org/10.3389/fpsyg.2016.01503 title= fpsyg.2016.01503] efficacious than antibodies acting only within one compartment.ResultsIn vivo studies 4E6 acutely improves cognition in htau mice and reduces soluble phospho-tau protein, whereas 6B2 does not affect cognition or tau levelsAt 11?2 months of age, the htau mice were assigned to control and treatment groups with similar cognitive (CFSM test) and motor status (Rotorod and Open Field). After baseline behavioral assessment, the mice received three antibody injections over a two week period and were re-evaluated on the same tests in addition to a fear conditioning test, followed by brain extraction for tissue analysis.BehaviorAs shown by trial errors, acute treatment with 4E6 led to significant improvements in spatial learning and memory in the CFSM test (48  fewer errors in posttest vs. pre-test; 13.6 (average errors) ?1.2 (SEM) to 7.1 ?0.9, p [https://dx.doi.org/10.3389/fnins.2013.00232 title= fnins.2013.00232] Closed Field Symmetrical (CFS) Maze (48  fewer errors, p