R markers certain to every single allele for BSGFV and BSIMV to

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-- Endogenous BSVs illuminate Musa balbisiana diversityABSVIG ORF2 ORF1kbPKW - eBSV carrierORFI II IIIBeBSGFV-7 eBSGFV-9 eBSOLV-BeBSIMVChromosome 1 eBSOLV-2 eBSIMV eBSIMV ChromosomeeBSGFVeBSOLVBABB hybrids with eBSV CpP (AcpP PPMO) or clpB (ClpB PPMO). Results shown are indicates allelesCBAAB hybrids with no infectious eBSV allelesDAAB hybrids with infectious eBSV allelesABAABeBSGFV-StressAABeBSGFV-eBSOLV-2 Chromosome 1 Chromosome 1 eBSIMV ChromosomeeBSOLV-1 eBSIMVChromosomeChromosomeChromosomeENo infectionNo infectionIR ORF I ORF IIIBSVORF IIIFIG. Preliminary genotyping final results indicated that PKW-related eBSVs are restricted strictly for the B genome.R markers precise to each and every allele for BSGFV and BSIMV to genotype PKW-related eBSVs in 1479-5868-9-35 M. balbisiana species. They initial established a microsatellite-based phylogeny of M. balbisiana diploids (BB), revealing handful of polymorphisms in between the six identified groups. Preliminary genotyping outcomes indicated that PKW-related eBSVs are restricted strictly towards the B genome. They may be strongly conserved; all BB genotypes tested optimistic for eBSGFV, even though eBSIMV was absent or mutated in some BB accessions. These results recommend an integration occasion shortly immediately after speciation of M. balbisiana in the genus Musa and ahead of intraspecies diversification (approx. 27? Mya) (Christelov et al., 2011). a Nonetheless, the absence of geographical information and also the limited number (20) of accessible M. balbisiana accessions preclude a full description of eBSV polymorphism and evolution on Musa species.Sampling of Musa diversity was primarily based on genotyping of 22 SSR nuclear markers inside a population of more than 500 accessions (Hippolyte et al., 2012). To improve the diversity of B genomes, this sampling was complemented with 23 new accessions which includes many recently collected M. balbisiana and interspecific AAB and ABB hybrids of interest. A sample of 77 accessions was thus defined [24 BB, 11 ABB, 26 AAB, 3 AB, six AA, seven outgroups (OG)] (Table 1) representative of (1) the two species at the origin of all cultivated bananas (M. acuminata and M. balbisiana), (2) diploid and triploid hybrids of those two species and (3) some other Musa species as outgroups. This sample was characterized by nuclear genome at the same time as eBSV insertions. Fresh leaf samples were kindly supplied by the in vivo germplasm collections jir.2012.0117 of CIRAD in Guadeloupe and the International Institute of Tropical Agriculture (IITA) in Nigeria; the INIBAP Transit Center (ITC) in Leuven (Belgium) supplied plantlets from in vitro germplasm collection. Each genotype was documented with its genome constitution and subgroup classification according to the present agro-morphological classification [IPGRI-INIBAP (Bioversity International, 2016)], and ploidy levels have been estimated by flow cytometry (Dolezel et al., 1997). Total genomic DNA was extracted from banana leaf tissue as outlined by the approach of Gawel and Jarret (1991). TheDuroy et al. -- Endogenous BSVs illuminate Musa balbisiana diversityABSVIG ORF2 ORF1kbPKW - eBSV carrierORFI II IIIBeBSGFV-7 eBSGFV-9 eBSOLV-BeBSIMVChromosome 1 eBSOLV-2 eBSIMV eBSIMV ChromosomeeBSGFVeBSOLVBABB hybrids with eBSV allelesCBAAB hybrids with no infectious eBSV allelesDAAB hybrids with infectious eBSV allelesABAABeBSGFV-StressAABeBSGFV-eBSOLV-2 Chromosome 1 Chromosome 1 eBSIMV ChromosomeeBSOLV-1 eBSIMVChromosomeChromosomeChromosomeENo infectionNo infectionIR ORF I ORF IIIBSVORF IIIFIG. 1. Schematic eBSV representation inside the seedy diploid PKW banana plant (BB) and interspecific hybrids (ABB, AAB). (A) Overview of eBSV inside PKW. Banana genomic sequences are in green. The BSV genome is represented in linear view with dark blue, light blue and red boxes indicating ORF1, ORF2, and ORF3 of the virus, respectively. The intergenic region is in black.