Values between left and right cerebellar nuclei in shams or in

Since the kindling protocol involves repetitive stimuli with interstimulus intervals of more than six hours, we chose to determine the FosB immunopositive cell 6-CarboxyfluoresceinMedChemExpress 6-FAM order SB-269970 density as a marker of neuronal activation. 3.1. FosB. FosB staining was not confined to the cell nucleus, as would be expected from a nuclear staining, but was seen in the cytoplasm as well (see Figure 1). Some areas of the brain are more involved in generalized tonic-clonic seizures than others [9, 22?4]. The degree of involvement can be immunohistochemically determined by markers for immediately early genes, such as c-fos, FosB, and FosB. C-Fos and FosB peak at two and six hours after a stimulus, respectively. FosB is a highly stable FosB isoform that persists in the brain for several weeks after an initial stimulus [25]. Since the kindling protocol involves repetitive stimuli with interstimulus intervals of more than six hours, we chose to determine the FosB immunopositive cell density as a marker of neuronal activation. Our results in sham rats reveal that basal expression of FosB is present in all deep cerebellar nuclei and that, among the deep nuclei, the DN contains the highest density of FosB immunopositive cells. The DN receives input from the more lateral parts of the cerebellar cortex while IN and FN receive input from the vermian cortex (for a review see Voogd [26]).Values between left and right cerebellar nuclei in shams or in kindled animals; therefore values from both hemispheres were pooled.Values between left and right cerebellar nuclei in shams or in kindled animals; therefore values from both hemispheres were pooled. 3.1. FosB. FosB staining was not confined to the cell nucleus, as would be expected from a nuclear staining, but was seen in the cytoplasm as well (see Figure 1). This "FosBlike immunoreactivity" was almost exclusively found in the most lateral regions of the deep cerebellar nuclei and was observed in the DN in particular. Density of FosB immunoreactive (FosB-ir) cells was lower in the DN and FN of kindled animals than in shams (DN 108.5 ?12.4 versus 152.1 ?7.7,