Ecies are intermingled within a species-independent manner (Supplementary Information Fig. S

Ecies are intermingled within a species-independent manner (Er {and the|and also the|as well as the|along Supplementary Information Fig. In contrast, comparisons of subfamily III sequences of these species and low-copy counterparts of subfamily III in other species examined show high interspecific sequence conservation plus the complete lack of any species-diagnostic mutations, and thus they seem to become intermixed inside the subfamily III clade (Supplementary Data Fig. S3). HinfI sequences of Carduncellus (subfamily IV) appear intermingled devoid of separation by distinct affinity (Supplementary Information Fig. S3). In the case of Rhaponticum and Klasea, sequences tend to be grouped by certain affinity (Supplementary Information Fig. S3). Sequences of subfamily VI of Volutaria are separated based on species of origin (Supplementary Information Fig. S3). However, the sequences of your two unique subfamilies discovered in Cheirolophus (VII and VIII) usually are not grouped in phylogenetic trees by certain affinity and appear to become intermixed (Supplementary Data Fig. S3). DISCUSSION HinfI sequences have been discovered to become present in the genomes of all the species analysed of subtribe Centaureinae. The differential spreading accompanied the two primary phases of radiation leading to two big groups in Centaureinae (Garcia-Jacas et al., 2001; Hellwig, 2004). Therefore, subfamilies V III are identified to prevail in older genera (initial phase of radiation inside the subtribe, late Oligocene iocene), despite the fact that a few repeats of subfamilies VI and VII had been isolated from Carduncellus and Centaurea (derived clade). Subfamilies I V have expanded predominantly in the genomes of species belonging for the derived clade of Centaureinae (second phase of radiation, Pliocene to Pleistocene). Notably, you will discover several species in the early diverging groups possessing subfamilies I II as the big representatives of HinfI sequences in their genomes. These information suggest that subfamilies I V have expanded lately, replacing other subfamilies in derived genera and in older genera. The replacement of one particular sequence variant by a further in diverse species is usually a common feature of satellite DNA that could be a consequence on the dynamics of satellite DNA evolution (Plohl et al., 2010, 2012). Molecular mechanisms of non-reciprocalDerived cladeIn phylogenentic analyses of subtribe Centaureinae (GarciaJacas et al., 2001), inside the derived clade, the Carthamus complicated occupies the earliest diverging position, and subgenera Jacea and Cyanus of Centaurea, for whi.Ecies are intermingled in a species-independent manner (Supplementary Data Fig. S3). Nonetheless, most sequences of subfamily III belonging to Phonus and Carthamus species tend to be grouped by taxonomic affinity, on 1 hand the sequences of Phonus arborescens and, on the other, the sequences of Carthamus tinctorius and these of Carthamus lanatus, despite the fact that you will discover some intermixed sequences from each and every (Supplementary Data Fig. S3). In contrast, comparisons of subfamily III sequences of these species and low-copy counterparts of subfamily III in other species examined show high interspecific sequence conservation plus the full lack of any species-diagnostic mutations, and for that reason they seem to become intermixed within the subfamily III clade (Supplementary Information Fig. S3). HinfI sequences of Carduncellus (subfamily IV) seem intermingled without having separation by distinct affinity (Supplementary Data Fig. S3). In the case of Rhaponticum and Klasea, sequences are likely to be grouped by precise affinity (Supplementary Data Fig.