Al replicates. (XLS)Table S9 Immunostaining images from Human Protein Atlas.

For every single gene, the table specifies its membership (MM) within the ciliary module in every dataset. Module membership was calculated as Pearson correlation involving expression profile of a gene and integrated expression profile on the ciliary module (see Solutions, section ``Expanding modules towards the genome scale). Green indicates that the gene belongs towards the journal.pone.0174724 ciliary module in a offered dataset. Genes that belong for the ciliary module and have MM worth .0.75 were considered hubs within the respective dataset. For any offered gene, statistical significance of MM difference between the tissues is described by ANOVA P-value. (2) The ``Tissue specificity spread sheet describes validation with the differentially coexpressed genes determined by the GSE7307 dataset. The table shows expression levels in the genes in 10 ``ependyma-positive brain samples, 7 airway samples and 3 fallopian tube samples. For every single gene, imply expression level in the tissues is provided. Tissue together with the GSK2256098MedChemExpress GSK2256098 highest expression level of every gene is colored depending on centrality status in the gene within this tissue. Specifically, green denotes that the gene represents a hub in this tissue's ciliary module, grey denotes that the gene represents a hub in a various tissue's ciliary module. For each and every gene up-regulated inside the similar tissue exactly where the gene represents a ciliary hub, statistical significance of up-regulation is offered (Student's t-test P-value immediately after a Benjamini-Hochberg correction). For genes supported as tissue-specific markers of ciliated cells, the underlying tissue specificity profile is visualized as a histogram. (3) The ``Protein Atlas spread sheet describes validation of the differentially coexpressed genes with immunostaining data. Human Protein Atlas contained data for three with the eight differentially coexpressed genes. Given that ependyma is absent from Protein Atlas, we compared the immunostaining data between airways (bronchus and nasopharynx) and fallopian tubes. (XLS)Table SAcknowledgmentsWe would like to thank Aliesa de Bray and Steven Kunnen in the Leiden University Medical Center for performing the immunofluorescent staining in the MDCK cell line.Author ContributionsConceived and developed the experiments: AI MS PTH DP WVR. Performed the experiments: WVR DP. Analyzed the information: AI. Contributed reagents/materials/analysis tools: PTH WVR DP. Wrote the paper: AI PTH DP MS. Before the 1990s there was incredibly tiny evidence of dinosaurs within the western half of Australia. That vast geographic area, roughly equivalent in area towards the western half of the continental USA, had made only a handful of reports of some three-toed dinosaur trac.Al replicates. (XLS)Table S9 Immunostaining pictures from Human Protein Atlas. Proteins are grouped by novelty categories: known ciliary proteins (category I), previously predicted candidates (category II) and novel candidates (category III). The summary in the leading of the document fnhum.2013.00596 briefly describes the staining pattern of every single protein within the tissues and specifies the resulting staining class. The immunohistochemical photos underlying the protein classification are offered beneath the summary table. The ``Protein column specifies protein name, antibody ID and novelty category of every single protein. The ``Airways and ``Fallopian tubes columns provide Protein Atlas photos for the respective tissues. Inside the ``AirwaysDifferential coexpression evaluation among the tissues. (1) The ``Differential coexpression spread sheet contains only the differentially coexpressed genes.