Figure represents a simplified tree layout without tip labels, indicating the

To get a detailed version of this tree, refer to Supplementary Data Fig. S3.Quesada del Bosque et al. -- HinfI satellite DNA evolution in Centaureinae exchange (unequal crossing-over, gene conversion, rollingcircle replication and Te, may possibly prolong re-insertion, and transposon-mediated exchange) would spread new sequence variants appearing in individual repeat units of a family members of sequences and the modifications are fixed inside a population of randomly mating folks by sexual reproduction based on a time-dependent two-step procedure referred to as molecular drive, which leads to concerted evolution ??(Plohl et al., 2010, 2012; Perez-Gutierrez et al., 2012). Satellite DNA modifications because of gradual accumulation of sequence divergence which final results in divergence of satellite sequences in reproductively isolated groups of organisms (Plohl et al., 2012). Nevertheless, provided the scattering of subfamilies in every single lineage of Centaureinae, an alternative but not mutually exclusive hypothesis could explain this differential distribution. In line with the `library' hypothesis, associated taxa share a library of various conserved satellite DNA sequences (diverse satellite DNA households but additionally monomer variants or subfamilies of a satellite DNA family members), which might be differentially .1371/journal.pgen.March 15,6 /Robust Identification of Soft and {Hard|Difficult amplified in each taxa. Variability can stay for extended evolutionary periods by reduced action of molecular mechanisms of non-reciprocal exchange, and sequence variants persist as a library (Mravinac et al., 2002; Mestrovic et al., 2006) from which any of them could possibly be differentially amplified in each taxon with all the subsequent replacement of one sequence variant by yet another in unique species. When this occurs, the study of unrelated species-specific dominant satellite DNA repeats reveals the presence of low-copy counterparts of every of them in other examined species, and comparisons of high-copy and low-copy monomer variants of these satellites show higher interspecific sequence conservation as well as the complete lack of any species-diagnostic mutations, as  ?identified in Palorus (Mestrovic et al., 1998). This hypothesis has  ?been proved in insects (Mestrovic et al., 1998; Mravinac et al., 2002; Cesari et al., 2003; Pons et al., 2004) and plants ?(Navajas-Perez et al., 2009; Quesada del Bosque et al., 2011), and could clarify the main observation created in Centaureinae concerning the scattering of HinfI varieties. The second clade incorporates 3 subclades, a single for every single of subfamilies VI III. In every single clade, sequences of subfamilies I, II or III on the distinctive sp.Figure represents a simplified tree layout without tip labels, indicating the correspondence between big clades and HinfI subfamilies. Bayesian posterior probability values for the key nodes are indicated. For a detailed version of this tree, refer to Supplementary Information Fig. S3.Quesada del Bosque et al. -- HinfI satellite DNA evolution in Centaureinae exchange (unequal crossing-over, gene conversion, rollingcircle replication and re-insertion, and transposon-mediated exchange) would spread new sequence variants appearing in person repeat units of a family of sequences along with the alterations are fixed within a population of randomly mating men and women by sexual reproduction based on a time-dependent two-step process called molecular drive, which results in concerted evolution ??(Plohl et al., 2010, 2012; Perez-Gutierrez et al., 2012). Satellite DNA modifications because of gradual accumulation of sequence divergence which final results in divergence of satellite sequences in reproductively isolated groups of organisms (Plohl et al., 2012).