Laimus had been 76, 21, 87, and 81 , respectively in Trial I

abbreviatus) emerging from soil, soil pH and moisture, citrus fibrous roots dry A manner constant with their anti-LGBT, purportedly religious beliefs {even weight, free-living (FLN) and plant-parasitic nematodes (PPN), and measurement by real-time PCR of 6 entomopathogenic nematode (EPN) species, 5 species of nematophagous fungi (NF), 2 Paenibacillus bacterial species which can be phoretic on EPNs and Acrobeloides-group nematodes that can compete with EPNs. The NF Paecilomyces lilacinus increased in plots where it was augmented (T1 P = 0.053; T3 P 0.001; T6 P = 0.021), reaching a maximum level at T3 that was 17.5-fold higher than that in controls. In the similar time (T3), two indigenous NF, Arthrobotrys dactyloides and Monacrosporium gephyropagum, tended to boost (P = 0.051 and P = 0.078, respectively) in P. lilacinusamended plots, as did the DNA recovered from nematode samples and citrus fibrous root weights (P = 0.003 and P = 0.022).Laimus had been 76, 21, 87, and 81 , respectively in Trial I; and have been 54, 51, 48, and 78 , respectively in Trial II. Confirmation of the feeding on Meloidogyne spp. and R. reniformis by Neoactinolaimus applying multiplex PCR evaluation is in progress. Additional research is needed on culture media that will support much more consistent reproduction of Neoactinolaimus, and an in vivo assay on suppression of Meloidogyne spp. and R. reniformis by Neoactinolaimus. MANIPULATING SOIL Food WEBS Inside a FLORIDA ORGANIC CITRUS ORCHARD TO Boost BIOCONTROL BY ENTOMOPATHOGENIC NEMATODES. Campos-Herrera, Raquel1,two, F. E. El-Borai1,3, L. W. Duncan1. 1 Citrus Study and Education Center, University of Florida, 700 Experiment Station Rd, Lake Alfred FL 33850; two Instituto de Ciencias Agrarias, CSIC, Serrano 115 dpdo Madrid, 28006, Spain; and 3Plant Protection Department, Faculty of Agriculture, Zagazig University, Egypt. An emerging organic citrus market in Florida could benefit tremendously from effective, nonconventional techniques to mitigate losses from pests and illnesses. We're investigating the effects of OMRI (Organic Supplies Evaluation Institute) approved cultural practices on soil food webs in order to develop solutions to conserve and boost biological handle of insect pests by indigenous entomopathogenic nematodes. An experiment was established inside a industrial, organic citrus orchard on Florida's central ridge. The treatment options consisted of three amendments: i) a mulch of commercially pelleted chicken manure, ii) a commercial formulation of Paecilomyces lilacinus applied twice, iii) sulfur applied twice to reduce soil pH, and iv) an unamended handle. The experimental style was complete randomized block with ten replications, every comprising three adjacent trees. Soil samples were taken pre-application and 1, 3 and 6 months post-treatment initiation (T0-T6). Response variables included adult root weevils (D. abbreviatus) emerging from soil, soil pH and moisture, citrus fibrous roots dry weight, free-living (FLN) and plant-parasitic nematodes (PPN), and measurement by real-time PCR of 6 entomopathogenic nematode (EPN) species, five species of nematophagous fungi (NF), 2 Paenibacillus bacterial species that are phoretic on EPNs and Acrobeloides-group nematodes which can compete with EPNs. The only EPNs detected had been Steinernema diaprepesi,456 Journal of Nematology, Volume 44, No. 4, December 2012 Heterorhabditis indica and H. zealandica. Seasonal enhance in numbers of H. zealandica (P 0.001) and Acrobeloidesgroup (P 0.001) have been not affected by therapies. All treatments decreased H. indica and Hirsutella rhossiliensis when compared with controls at T1 (P = 0.040, and 0.019, respectively). Numbers of Paenibacillus sp. have been directly related to both those of S.