N the species of Carthamus and Phonus, we discovered only HinfI

Nonetheless, some species of this genus also have low-copy At they had been competent for mitochondrial respiratory repeats of Title Loaded From File subfamilies III and VI in their genomes. We obtained results comparable to these obtained using the general primers. Nonetheless, some differences were identified (Fig. 2; Supplementary Information Fig. S2). As an example, subfamily I is present in Centaurea granatensis and Crupina vulgaris (the only species of Centaurea and Crupina for which we did not receive repeats of subfamily I right after sequencing of amplified solution employing the primers CenHinf1 and CenHinf2). The subfamily III is located in species of Volutaria and Cheirolophus besides those previously detected with all the `general' primers. Nevertheless, the primers used have been not able to amplify repeats of subfamily III in Carduncellus (although we detected some repeats of this kind using the CenHinf1 and CenHinf2 primers). Subfamily V is found not merely in Klasea and Rhaponticum but additionally in Volutaria, as we previously found by sequencing the CenHinf1/CenHinf2 amplified product. Subfamily VI was located only in Volutaria, as detected by CenHinf1 and CenHinf2 primers. Phylogenetic reconstruction by ML and Bayesian inference resulted in similar trees. A Bayesian majority-rule consensus tree with Bayesian PPs is shown in Supplementary Data Fig. S3. A simplified tree layout without having tip labels is shown in Fig. 3. The trees didn't group sequences by precise or generic affinity. Although the tree grouped collectively the HinfI monomers of some particular genera or subtribal group, essentially the most widespread view is the fact that the repeats are grouped by HinfI subfamilies, which are conserved amongst diverse species, as classified in Figs 1 and two. The tree represented in Fig. three shows indications of the correspondence involving clades and HinfI subfamilies. We can distinguish two0? Subfamily VII 1 1 0?306 1 Subfamily VI0?997Subfamily VIII0? 1 0?two 1? Subfamily VSubfamily IV 0?947 0?997 0?0?677 1 1 0?672 1 0?577 0?276 0?621 1 1 Subfamily III1 0?67 1 Subfamily I0? 0?997 Subfamily II 0?0?F I G .N the species of Carthamus and Phonus, we identified only HinfI variety III sequences. Subfamily IV is characteristic of Carduncellus. Nevertheless, some species of this genus also have low-copy repeats of subfamilies III and VI in their genomes. Subfamily V was located in Rhaponticum and Klasea. These species had only this kind of HinfI repeat, except R. acaule, which also contained sequences of subfamilies II, IV and VI in its genome. We analysed 4 species of Volutaria (Table 1). The HinfI sequences of V. lippii belonged to subfamilies I, II, III or V, primarily to sort III (nine sequences out of 14). Also, most HinfI sequences of V. crupinoides belonged to subfamilies II, III and IV. However, the majority of the sequences of V. muricata and V. tubuliflora and 3 of V. crupinoides belonged to subfamily VI. Three species of Cheirolophus (C. intybaceus, C. sempervirens and C. sventenii) shared two sorts of HinfI monomers in their genomes, those of subfamilies VII and VIII. Cheirolophus sventenii also had a variety II repeat. Cheirolophus falcisectus had only sequences of subfamily VII. In contrast, among the sequences isolated from C. teydis, 12 belonged to subfamily III along with the other 3 to subfamily II (Fig.