Ree Short Life (PTSL) involves the

1Plant Pathology Dept., University of Arkansas, Fayetteville, AR 72701; and 2North Carolina Department of Agriculture Customer Services, Nematode Assay SGC707 web Section, 4300 Reedy Creek Road, Raleigh, NC 27607-6465. Results of this test are still to become determined.Ree Quick Life (PTSL) includes the interaction of environmental situations (winter temperature, soil history, early pruning) and pathogens ring nematode (Mesocriconema xenoplax) and Pseudomonas syringae.Meeting Abstracts 487 PTSL is characterized by premature tree death by bacterial canker or cold injury, resulting in substantial financial losses, which have been observed within the Southeastern U.S. for the past 40+ years. Early research showed that rootstock impacted PTSL incidence, and also a collaboration of Clemson University and USDA-ARS lead to the release of a additional tolerant rootstock trademarked Guardian`BY520-9'. To investigate the genetic and molecular basis for this tolerance, Nemaguard, a PTSL susceptible rootstock, and Guardianselection 3-17-7 have been crossed. The F1 plants were self pollinated to create segregating F2 populations. The trees had been rated annually, from 2004 by way of 2008, for their response to PTSL. A single hundred and seventy-six microsatellite/Simple Sequence Repeat (SSR) markers, every mapping to a precise chromosomal location on the Prunus reference nuclear genome, have been utilised to screen the two parents and F1-11. Only 53 markers showed polymorphism amongst the parents, and had been heterozygous in F1-11. These SSRs had been then screened on the F2-11 population (N=100). Segregation information for PTSL-response and SSR marker inheritance have been complied and subjected to Evaluation of Variance to determine differences in Genotypic Implies (GMs). Nine SSR loci correlated using a response to PTSL. These nine SSR loci had been distributed on four linkage groups (e.g., Linkage Group-1, LG-2, LG-4 and LG-6). Four in the nine loci (EPDCU5100, Pacita 27, UDA008 and UDA029) accounted for substantially of your variance within the trait based upon their huge GMs (>2.five). Immediately after making a molecular map from the segregation information, QTL analysis identified a quantitative locus on LG2 that was crucial in all 5 years in the study. Also, this chromosomal interval contained three from the four SSR marker loci that accounted for the majority from the variation. These final results identified regions with the peach genome exactly where genes controlling PTSL susceptibility and tolerance reside, hence assisting to define the genetic basis for response to PTSL as a complicated but tractable trait. Disease resistant genes found in the region on the most promising PTSL-associated QTL might be discussed. HOST Variety ADDITIONS FOR HETERODERA URTICAE AND CACTODERA BETULAE FROM ARKANSAS. Robbins1, Robert T., and Weimin Ye2. 1Plant Pathology Dept., University of Arkansas, Fayetteville, AR 72701; and 2North Carolina Department of Agriculture Consumer Services, Nematode Assay Section, 4300 Reedy Creek Road, Raleigh, NC 27607-6465. In January, 2012, samples had been taken from a variety of winter weeds at Toad Suck Park on the Perry County bank in the Arkansas River near Conway, Arkansas. Cysts of Heterodera urticae, that was initially reported at the 2011 SON meetings, have been present in substantial numbers. Crucial inspection of these plant roots revealed white females attached towards the roots of typical chickweed (Stellaria media (L.) Vill. Quite a few juveniles were identified inside the soil related with chickweed, but males weren't present. A number of species of winter weeds were inoculated with juveniles obtained from cysts recovered from the soil to determine other attainable hosts.