Values between left and right cerebellar nuclei in shams or in

This "FosBlike immunoreactivity" was order SB-269970 almost exclusively found in the most lateral regions of the deep cerebellar nuclei and was observed in the DN in particular. Since the kindling protocol involves repetitive stimuli with interstimulus intervals of more than six hours, we chose to determine the FosB immunopositive cell density as a marker of neuronal activation. To the best of our knowledge, no data are available in literature on basal cerebellar FosB levels nor on cerebellar FosB expression in epilepsy. Our results in sham rats reveal that basal expression of FosB is present in all deep cerebellar nuclei and that, among the deep nuclei, the DN contains the highest density of FosB immunopositive cells. The DN receives input from the more lateral parts of the cerebellar cortex while IN and FN receive input from the vermian cortex (for a review see Voogd [26]). Our data suggest that, in shams, the vermian cortex has a stronger inhibitory effect on its target nuclei than the cortex of the cerebellar hemispheres. Furthermore, Fos immunoreactivity has been described to occur in three subcellular expression patterns, that is, solelynuclear, solely cytoplasmic, and both [27]. We found cells with nuclear FosB immunoreactivity as well as cells with cytoplasmic staining. This phenomenon has been described for the hippocampus, dentate gyrus, and amygdala by others [28] and appears to be present in the deep cerebellar nuclei as well.Values between left and right cerebellar nuclei in shams or in kindled animals; therefore values from both hemispheres were pooled. 3.1. FosB. FosB staining was not confined to the cell nucleus, as would be expected from a nuclear staining, but was seen in the cytoplasm as well (see Figure 1). This "FosBlike immunoreactivity" was almost exclusively found in the most lateral regions of the deep cerebellar nuclei and was observed in the DN in particular. Density of FosB immunoreactive (FosB-ir) cells was lower in the DN and FN of kindled animals than in shams (DN 108.5 ?12.4 versus 152.1 ?7.7,